Concentrated
Taq DNA Polymerase
Certain
types of PCR, such as RAPD PCR, require high concentrations of
Taq DNA Polymerase. However, adding larger volumes of Taq DNA Pol
will increase the glycerol concentration in the reaction mix (Taq
DNA Polymerase storage buffer contains 50% glycerol to prevent
freezing) and high glycerol levels can interfere with PCR. To
avoid inadvertently raising glycerol concentrations in the final
mix, Taq DNA Polymerase at 15 U/l concentration is recommended.
Unit definition
One unit is the amount of enzyme required to catalyze the
incorporation of 10 nanomoles of nucleosides into acid insoluble
material per 30 min. at 74C under assay conditions. 10x T.Pol
incubation buffer with/without MgCl2 and a 25mM
solution MgCl2 are provided.
Storage
conditions
20mM Tris-HCl pH 8.0, 100mM KCl,
0.1mM EDTA, 1mM dithiothreitol,
0.5% Tween 20, 0.5% Nonidet P40,
50% glycerol.
Incubation
buffer (1X)
10mM Tris-HCl pH 9.0, 50mM KCl,
1.5mM MgCl2, 0.1% Triton X-100,
0.2 mg/ml BSA
Quality
control
Performed on every lot of enzyme:
specific PCR on genomic and phage templates, activity check,
absence of ribonucleases, absence of nickases and endonucleases,
absence of 3' exonucleases, purity on SDS-PAGE, absence of
proteases.
| Cat No. |
Description |
Size |
| EPTQC060 |
Taq
DNA Polymerase at 15U/l |
600U |
| EPTQC200 |
Taq
DNA Polymerase at 15U/l |
2000U |
Storage:
Store at -20C
10X
incubation buffers, one with and one without MgCl2, are provided.
A solution of 25mM MgCl2 is also supplied.
 Patent Information
for PCR Process
|
