- Synthesis
full length copies of cDNA ›10kb
- Low RNase H
activity
- Recombinant
enzyme
M-MuLV
Reverse Transcriptase, a recombinant enzyme, is an RNA-dependent
DNA polymerase. It is a single polypeptide with an apparent
molecular weight of 71 000 daltons. It requires Mg2
for optimal activity. As M-MuLV RT has a lower RNase H activity
than AMV reverse transcriptase, it is a better choice for
synthesising full-length copies of large mRNA (in excess of 10
kb), under optimal reaction conditions.
Storage
Tris HCl 50mM, pH 8.3, NaCl 0.1M, EDTA 1mM, DTT 5mM, Triton X-100
0.1%, Glycerol 50%(v/v). Temperature -20C.
Incubation
buffer
Tris HCl 50mM, pH 8.3, KCl 75mM, MgCl2 3mM, DTT 10mM.
Unit
definition
One unit incorporates one nmol of TMP into DE-81 adsorbable form
in 10 minutes at 37C using polyA-oligo dT12-18 as a substrate.
Purity
Free of contaminating, non-specific double-stranded and
single-stranded endonucleases, exonucleases and ribonucleases.
Typically greater than 90% pure by SDS-polyacrylamide gel
electrophoresis.
Activity
200 units/l.
cDNA synthesis activity: M-MuLV RT exhibits superior stability
during incubation at 37C. The amount of enzyme required is
generally only around 10 units per g of mRNA.
| EMMLV100 |
Moloney
Murine Leukemia Virus RT |
10 000 U |
| EMMLV103 |
Moloney
Murine Leukemia Virus RT |
30 000 U (3 x
10 000 U) |
| Supplied
with 5X incubation buffer |
 Patent Information
for PCR Process
Related products
First Strand Synthesis Opti-Kit
Two-Step RT-PCR-&GO Kits
|
