Polymerases for PCR

The need for innovative application-specific thermostable DNA polymerases is growing rapidly. Qbiogene has developed a comprehensive selection of enzymes covering all major PCR applications. Several of these polymerases are completely unique and offer important advantages. The tables below describe the major features and benefits of each enzyme.

Taq DNA Polymerase	For standard PCR applications – highly reliable, well-established and widely used polymerase.
Q-BioTaq DNA Polymerase	Recombinant truncated form of Taq DNA Polymerase lacking 5’-3’exo activity.
SurePrime Polymerase	Chemically modified Taq DNA Polymerase for convenient "hot start" PCR.
Isis DNA Polymerase™	Highly accurate, high thermostability proofreading recombinant DNA Polymerase originally isolated from Pyrococcus abyssii. Error rate is approximately 1 per 1.6 million base pairs per PCR cycle.
Arrow Taq™ DNA Polymerase	High performance mix for high sensitivity and long template amplification. Accuracy sufficient for most cloning needs.

Quality Control
To ensure the highest standard of quality, each lot of thermostable DNA polymerase is checked for activity, function and purity. Each thermostable polymerase is shipped with a lot-specific quality control datasheet.

Absence of nickases
Confirmed by incubating increasing amounts of enzyme with supercoiled plasmid DNA (pBR322). The maximum number of units that results in no relaxation of the supercoiled DNA, as visualized on an agarose gel, is stated on the lot-specific data sheet.

Absence of endonuclease contamination
Verified by an assay with Hind III/EcoR I fragments of DNA. Increasing amounts of enzyme are incubated with constant amount of substrate under appropriate incubation conditions. The maximum number of units that results in no degradation of the fragment pattern on agarose gel electrophoresis is stated on the lot-specific data sheet.

Absence of 3' exonuclease activity
Confirmed on 32P Klenow labeled Hind III fragments of DNA. After incubation under appropriate conditions, the absence of released radioactive phosphate is checked by means of DE-81 adsorption.
Absence of 5' exonuclease and 5' phosphatase activity

Demonstrated on 32P polynucleotide kinased Hind III fragments of DNA. After incubation under appropriate conditions, the absence of released radioactive phosphate is checked by means of DE-81 adsorption.

Absence of ribonucleases
Checked on 32P labelled RNA. After incubation under appropriate conditions, the absence of released 32P is checked using DE-81 adsorption.

PCR assay 1
Carried out using human genomic DNA as a template (ß-globin gene) with decreasing amounts of template DNA and decreasing units of DNA polymerase. A specific PCR product of 420 bp must be obtained.

PCR assay 2
Carried out using phage DNA as a template ( DNA) with decreasing amounts of template DNA and decreasing units of DNA polymerase. A specific PCR product of 500 bp must be obtained.

Purity
Verified by SDS-PAGE.

Patent Information for PCR Process

Patent Information for PCR Process