Q-BioTaq DNA Polymerase

Q-BioTaq DNA Polymerase

Recombinant, truncated form of Thermus aquaticus
Highly thermostable (98°C)
No 5'-3' exonuclease activity
Enzyme cannot degrade the 5' end of primers

Q-BioTaq DNA Polymerase is a novel thermostable DNA polymerase which can be used effectively for PCR and DNA sequencing by the chain termination method. This enzyme is encoded by a modified form of the Thermus aquaticus DNA polymerase gene with a N-terminal deletion. The properties of Q-BioTaq DNA Polymerase include high thermostability and absence of 5'-3' exonuclease activity. The enzyme is highly purified and free of RNases and endo- and exonucleases. The absence of 5'-3' exonuclease activity and high thermostability of this enzyme make it particularly useful in applications where multiple amplifications are required.

Cat No.	Product	Size
EPQBT010	Q BioTaq 5U/µl*	100U
EPQBT025	Q BioTaq 5U/µl*	250U
EPQBT050	Q BioTaq 5U/µl*	500U
EPQBT100	Q BioTaq 5U/µl*	1000U
EPQBT015	Q BioTaq 5U/µl*	5 x 100U

Two different 10X incubation buffers are provided, either with or without MgCl₂, MgCl₂, 25mM is supplied in a separate tube.

*The concentration of Q-BioTaq is in Klentaq units.
1U Klentaq = 4U DNA Taq DNA pol

One unit is the amount of enzyme required to catalysethe incorporation of 10 nanomoles of nucleosides into acidinsoluble material per 30 min. at 72°C under assay conditions.

Storage
50mM (NH₄)₂SO₄, 20mM Tris. HCl (pH 8.55 at 25°C), 0.1mM EDTA, 10mM mercaptoethanol, 0.5% Thesit and 50% glycerol. At -20°C.

Incubation Buffer 1x
50mMTris. HCl (pH 9.1 at 25°C), 0.15% BSA, 16mM (NH₄)₂SO₄ and 3.5mM MgCl₂.

Quality control
Activity, SDS-PAGE/purity, nuclease, specific PCR on genomic and phage templates

Patent Information for PCR Process

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