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T4
bacteriophage gene 32 Product (gp32)
- Enhances DNA
polymerase proofreading activity and PCR fidelity
- Increases
yield of large PCR DNA fragments
 T4 bacteriophage
gene 32 product, or gp32, is a non-sequence specific,
single-strand binding-protein isolated from the bacteriophage T4.
It is reported to act by binding to single-stranded DNA in a
co-operative manner thereby destabilizing double-stranded DNA
(1).
Used
with either Tfu or Arrow Taq™ DNA polymerase, gp32 protein
strongly increases PCR fidelity (table page 58). The gp 32
protein is used to improve yields of long PCR products catalyzed
by Taq DNA Polymerase (2), DNA polymerase proofreading (3) and
amplification efficiency in low purity DNA. It has also been used
to stabilize single-stranded regions of DNA for site-directed
mutagenesis.
Storage
Tris-HCl 20mM, pH 8.0, NaCl 100mM, EDTA 1mM, DTT 0.5mM, Glycerol
50%. At -20C.
The
supplied protein is a purified recombinant form free of
endonucleases and nickases.
| Cat No. |
Size |
| TGP32100 |
100 g |
References
(1) Topal, M.D. and Sinha, N.K. (1983). Products of bacteriophage
T4 genes 32 and 45 improve the accuracy of DNA replication in
vitro. J. Biol. Chem. 258: 12274-12279.
(2) Schwarz, K., Hansen-Hagge, T., Bartram, C. (1990). Improved
yields of long PCR products using gene 32 protein. Nucleic Acids
Research 18 :1079.
(3) Sandhu, D.K., Keohavong, P. (1994). Effects of the T4
bacteriophage gene 32 product on the efficiency and fidelity of
DNA amplification using
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