bacteriophage gene 32 Product (gp32)
T4 bacteriophage gene 32 product, or gp32, is a non-sequence specific, single-strand binding-protein isolated from the bacteriophage T4. It is reported to act by binding to single-stranded DNA in a co-operative manner thereby destabilizing double-stranded DNA (1).
Used with either Tfu or Arrow Taq™ DNA polymerase, gp32 protein strongly increases PCR fidelity (table page 58). The gp 32 protein is used to improve yields of long PCR products catalyzed by Taq DNA Polymerase (2), DNA polymerase proofreading (3) and amplification efficiency in low purity DNA. It has also been used to stabilize single-stranded regions of DNA for site-directed mutagenesis.
The supplied protein is a purified recombinant form free of endonucleases and nickases.