Taq DNA Polymerase

Taq DNA Polymerase

Recombinant form - for general PCR applications

5'-3' exonuclease activity
No 3'-5' proofreading activity
Highly reproducible results
Ideal for T/A cloning

Reference Library
	Taq-&GO PCR Mastermix

Highly processive, recombinant, thermostable 5'-3' DNA polymerase catalyzes the addition of mononucleotide units to the 3'-OH end of a primer chain. Remains functional even after prolonged incubation at 95C. Applications include PCR, RTPCR, DNA sequencing and DNA labeling. This enzyme possesses 5’-3’ exonucleases activity.

Unit definition
One unit is the amount of enzyme required to catalyze the incorporation of 10 nanomoles of nucleotides into acid insoluble material in 30 min. at 74C under assay conditions.

Storage conditions
20mM Tris-HCl pH 8.0, 100mM KCl,
0.1mM EDTA, 1mM dithiothreitol,
0.5% Tween 20, 0.5% Nonidet P40, 50% glycerol. Stored at -20° C.

Incubation buffer with MgCL₂(1X)
10mM Tris-HCl pH 9.0, 50mM KCl,
1.5mM MgCl₂, 0.1% Triton X-100,
0.2 mg/ml BSA

Incubation buffer w/o MgCL₂(1X)
10mM Tris-HCl pH 9.0, 50mM KCl,
0.1% Triton X-100, 0.2 mg/ml BSA

Figure a:
Amplification of a 400 bp fragment of human ß globin with Taq DNA polymerase. Varying amounts of human ß globin, 1 pg to 100 ng, were amplified under the following conditions: 50 µl reaction; 50 pmoles each specific primer, 300 µM each dNTP, 1U Taq DNA pol, MgCl2 1.5 mM, 5 min at 93°C then 1 min at 91°C, 1 min at 62°C, 2 min at 70°C for 37 cycles, 1 min at 91°C, 1 min at 62°C and 10 min at 70°C for 1 cycle.Lane 1: marker pBR322/HaeIII, Lane 2: 1pg, Lane 3: 25 pg, Lane 4: 250 pg, Lane 5: 1ng, Lane 6: 10 ng, Lane 7: 100 ng, of template DNA respectively.

Figure b:
Amplification of a 1,600 bp DNA insert in pUC with Taq DNA polymerase. Varying amounts of pUC containing a cloned insert 1 pg to 1 ng were amplified under the following conditions: 50 µl reaction; 50 pmoles each specific oligo, 300 µM each dNTP, 1U Taq DNA pol, MgCl2 1.5 mM, 30 sec at 94°C, 1 min at 68°C, 2 min at 70°C for 37 cycles, 1 min at 94°C, 1’ at 68°C and 10 min at 70°C for 1 cycle. Lane 1: 1pg, Lane 2: 10pg, Lane 3: 100pg, Lane 4: 1ng, of template DNA respectively.

Quality control
Performed on every lot of enzyme:
specific PCR on genomic and phage templates, activity check, absence of ribonucleases, absence of nickases and endonucleases, absence of 3' exonucleases, purity on SDS-PAGE, absence of proteases.

Cat No.	Concentration	Pack Size	Incubation buffer included
EPTQA025	5U/l	250U	10 x Buffer with MgCl₂ 10 x Buffer w/o MgCl₂ 25mM MgCl₂ sol.
EPTQA325	5U/l	3 x 250U	10 x Buffer with MgCl₂ 10 x Buffer w/o MgCl₂ 25mM MgCl₂ sol.
EPTQA925	5U/l	10 x 250U	10 x Buffer with MgCl₂ 10 x Buffer w/o MgCl₂ 25mM MgCl₂ sol.
EPTQD025	5U/l	250U	Only 10 x Buffer with MgCl₂
EPTQD325	5U/l	3 x 250U	Only 10 x Buffer with MgCl₂
EPTQD925	5U/l	10 x 250U	Only 10 x Buffer with MgCl₂
EPTQA100	5U/l	1 000U	10 x Buffer with MgCl₂ 10 x Buffer w/o MgCl₂ 25mM MgCl₂ sol.
EPTQA105	5U/l	5 x 1 000U	10 x Buffer with MgCl₂ 10 x Buffer w/o MgCl₂ 25mM MgCl₂ sol.
EPTQA500	5U/l	5 000U	10 x Buffer with MgCl₂ 10 x Buffer w/o MgCl₂ 25mM MgCl₂ sol.
EPTQBUF1		5 x 1 ml	10 x Buffer with MgCl₂
EPTQBUF2		5 x 1 ml	10 x Buffer w/o MgCl₂ 25mM MgCl₂ sol.

Storage: Store at -20C

Patent Information for PCR Process

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