Separate Mastermixes for RT and PCR (Standard and High Fidelity)
- For reproducible high yield amplification
- Optimized ready-to-use RT and PCR Mastermixes
- Choice of standard or high fidelity PCR mastermix
- Significant savings in preparation time
- Reduced risks of errors and contamination
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Reference Library
 Brochure
Manual |
RT-PCR (reverse transcription-polymerase chain reaction) is a common technique used for the detection and study of mRNA. Two-step RT-PCR allows each part of the process to be individually optimized for yield and permits multiple PCRs from a single RNA sample.
Qbiogene's Two-Step RT-PCR Kits provide two ready-to-use Mastermixes: a Master Mix for the Reverse Transcription step (RT-&GO™ ) and a Mastermix for the PCR (Taq-&GO™ or Isis -&GO™ High Fidelity). &GO™ Mastermixes are ready to use, containing all the required components for RT and PCR with the exception of the template and specific primers.
This new kit concept allows you to enjoy the flexibility of Two-Step RT-PCR in the same time as it takes to do one-step RT-PCR. For the PCR step, the kits offer the choice between 2 Mastermixes containing either Qbiogene's Taq DNA polymerase for standard PCR (Taq-&GO™ ) or the high fidelity Isis DNA Polymerase (Isis-&GO™ ) when, for example, the further cloning of the PCR product is forseen.
| Kit Components |
|
Quantity |
RT-&GO™ Mastermix (M-MuLV Reverse transcriptase,
RNase inhibitor, DTT, dNTPs) |
|
800 µl |
| Anchored Oligo (dT)25V (40 µM) |
|
100 µl |
| N8 random octamers (40 µM) |
|
100 µl |
| 100 mM DDT |
|
500 µl |
| Water DEPC treated |
|
1 ml |
| Taq-&GO™ or Isis-&GO™ Mastermix |
|
1 ml |
| (100 U polymerase, buffer, dNTPs) |
|
|
(Taq-&GO™ or Isis -&GO™ Mastermixes can be stored at 4 ° C) Storage: -20 ° C
| RT2ST100 |
Standard Two-Step RT-PCR-&GO™ Kit |
100 reactions |
| RT2HF100 |
High Fidelity Two-Step RT-PCR-&GO™ Kit |
100 reactions |
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