New! PhoenIX Filter Maxiprep Kit

Unique filter device for immediate lysate clearing Fits within binding column No extra preparation or incubation No inconvenient syringes No danger of clogging Patented ion exchange resin and optimized buffer system offers: Increased binding capacity for higher yield Superior separation of unwanted molecules during wash step Elution of ultrapure plasmid equal to 2 passes through CsCl Consistent, reproducible results from column to column Fast protocol that saves at least an hour over similar kits Purification of constructs up to 150 kb Safe procedure with no hazardous materials Low endotoxin levels allow transfection without additional purification Pre-packed columns flow at a consistent, even rate with no clogging Low price and flexibility add value to lab budgets and procedures

Reference Library   Protocol - Filter Maxiprep Kit

Each of the 10 preps in the PhoenIX Filter Maxiprep Kit provides 500 µg of ultrapure plasmid DNA from 100 - 500 ml of bacterial culture. Cleared bacterial lysate is poured through the filter onto the PhoenIX Maxi Column for rapid purification by gravity flow. Protocols for high copy and low copy plasmids are included in the instruction manual. If all 10 columns are to be used for low copy preps, the PhoenIX Midiprep Low Copy Refill Kit is available to replenish the alkaline lysis buffers.

Kit Components: RNase A, Cell Resuspension Buffer, Lysis Buffer, Neutralization Buffer, PhoenIX Filter Maxi Columns, Disposable Maxi Column Rack, Equilibration Buffer, Column Wash Buffer, Elution Buffer.

• PhoenIX™ Midiprep Kit
• PhoienIX™ Maxiprep Kit
• PhoenIX™ Gigaprep Kit

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