PhoenIX Gigaprep Kit

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• Patented ion exchange resin and optimized buffer system offers:
  • Increased binding capacity for higher yield
  • Superior separation of unwanted molecules during wash step
  • Elution of ultrapure plasmid equal to 2 passes through CsCl
  • Consistent, reproducible results from column to column
  • Purification of constructs up to 150 kb
  • Safe procedure with no hazardous materials
• Low endotoxin levels allow transfection without additional purification
• Pre-packed columns flow at a consistent, even rate with no clogging
• Low price and flexibility add value to lab budgets and procedures

Each of the 5 preps in the PhoenIX Gigaprep Kit provides 10 mg of ultrapure plasmid DNA from 2.5 5.0 L of bacterial culture. Cleared bacterial lysate is prepared by vacuum filtration and loaded onto the PhoenIX Giga Column for rapid vacuum-driven purification.

Kit Components: RNase A, Cell Resuspension Buffer, Lysis Buffer, Neutralization Buffer, Lysate Filtration Cartridges, PhoenIX Giga Columns, Equilibration Buffer, Column Wash Buffer, Elution Buffer.

• PhoenIX™ Midiprep Kit
• PhoenIX™ Maxipep Kit
• PhoenIX™ Filter Maxiprep Kit

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