CytoPure™ Transfection Reagent

First reagent to biodegrade upon delivery into cell Superior transfection efficiency in a broad range of cell lines Effective in presence or absence of serum Low toxicity Easy-to-use and supplied with an optimized protocol

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New Biodegradable Transfection Reagent

CytoPure™ is a revolutionary new reagent for transfection of plasmid DNA into a broad range of mammalian and insect cells. CytoPure™ is the first transfection reagent on the market proven to biodegrade into small, non-toxic molecules upon entry into the cell. Derived from a proprietary cationic polymer, the biodegradable nature of CytoPure™ increases the transgene expression and reduces the cellular cytotoxicity typically caused by large cationic polymers. The increased viability of transfected cells makes CytoPure™ the optimal choice for researchers trying to establish stable cell lines.

Biodegradation Mode of Action

Illustrated in Figure 1, the cationic polymers of CytoPure™ function by associating with negatively charged nucleic acid molecules to form a polyplex having a net positive charge. During endocytosis, the cationic polymers within the polyplex protect the nucleic acid and later facilitate release through the nuclear membrane. While other cationic polymers tend to accumulate in the cell nucleus resulting in high toxicity, CytoPure™ polymers biodegrade into small, non-toxic molecules which promote increased cell survival and overall cell health.

Figure 1 CytoPure™ biodegradation mode of action. 1. Attachment and endocytosis; 2. Endosome escape; 3. Nuclear localization; 4. CytoPure™ degradation and DNA release

Figure 2 Analysis of CytoPure™ polymer biodegradation into small, non-toxic particles. A.  GFP tracking of reagent biodegradation in 293 cells post-transfection B. GPC analysis of molecular weight decrease of CytoPure™ polymer over time

Figure 3 Viability of 293 cells 24 hours post-transfection measured by MTT assay

Figure 4 HUVEC cell condition post transfection with CytoPure™:DNA ratio of 14:1

Figure 5 Visualization of eGFP in HUVEC post transfection with CytoPure™:DNA ratio of 14:1, exhibiting close to 50% transfection efficiency

Table 1
Typical transfection efficiencies and cell cytotoxicity values obtained in various cell lines using CytoPure™

Available in Two Formulations for Optimization

CytoPure™ is available in two formulations: CytoPure™ -cmn is suited for a broad range of mammalian cells such as 293T, Cos 7, and HeLa. The second formulation is CytoPure™ - huv, which has been specifically designed for HUVEC and other primary and difficult to transfect cells. CytoPure™ reagents are easy to use and are suitable for both first time users and those experienced in transfection.

Stability & Storage: CytoPure™ is stable for 1 year when stored at 4°C