The sucrose gradient purification of the amplified baculovirus is essential for any in vivo experiments. This step ensures the removal of defective particles and contaminants remaining from the Sf9 culture media, and concentrates the baculovirus to high titers. Time required: 1 - 2 weeks (depending on amplification volume).
- Purifying the baculovirus on a sucrose cushion
- Dialyzing the purified baculovirus in a buffer suitable for long-term storage and aliquoting
Next step: Time-Course and MOI assay